Isolation and Identification of Secondary Metabolite Compounds from Ethyl acetate Extract of Salix tetrasperma Roxb. Leaves

Authors

  • Ria Januarti PTKI Medan
  • Sry wahyuni PTKI Medan
  • Dinda Rizka Fadhillah PTKI Medan
  • Trisno Afandi PTKI Medan

DOI:

https://doi.org/10.68115/reprokimia.v1i2.80

Keywords:

Salix tetrasperma, spektroskopi, flavonoid

Abstract

The aim of this study was to isolate and identify secondary metabolites from the ethyl acetate extract of dalu-dalu (Salix tetrasperma Roxb.) leaves. Isolation of secondary metabolites was carried out by maceration method and continued to column chromatography which was then identified by UV-Visible and FT-IR spectroscopy. The results of the phytochemical test showed that dalu-dalu leaves were positive for containing compounds that classified into the class of flavonoids, phenolics, terpenoids, steroids, and saponins. The yellow solid of the isolated compound has a melting point of 261-2620C. The test results for the isolated compound showed one yellow stain on the TLC plate with UV 365 nm and concentrated H2SO4. UV-Vis spectroscopy showed the absorption band at λmax 209 nm, 268 nm (benzoyl) and λmax 342 nm (cinamoil) which is a typical spectrum for flavonoid compounds. The IR spectrum of the isolated compound shows the absorption of the aromatic C=C, the carbonyl C=O, the aliphatic C-H, and the hydroxyl functional groups. The characterizations clearly mentioned that the isolated compound is a flavonoid compound substituted for the OH group.

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Published

2023-01-04

How to Cite

Januarti, R., Wahyuni, S. ., Fadhillah, D. R. ., & Afandi, T. . (2023). Isolation and Identification of Secondary Metabolite Compounds from Ethyl acetate Extract of Salix tetrasperma Roxb. Leaves. Jurnal Rekayasa, Teknologi Proses Dan Sains Kimia (REPROKIMIA), 1(2), 97–101. https://doi.org/10.68115/reprokimia.v1i2.80

Issue

Vol. 1 No. 2 (2022): Desember : Jurnal Rekayasa, Teknologi Proses dan Sains Kimia

Section

Volume 1 No 2 Tahun 2022

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